CD59 (CD59) antibody (APC)

Antigen: CD59 (CD59)

Synonyms: 1F5, EJ16, EJ30, EL32, G344, MIN1, MIN2, MIN3, MIRL, HRF20, MACIF, MEM43, MIC11, MSK21, 16.3A5, HRF-20, MAC-IP, p18-20, MGC2354, FLJ38134, FLJ92039

Clonality: Monoclonal (MEM-43)

Host: Mouse

Reactivity: Human

Conjugate: APC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN125700

Additional Information

Alternative name: CD59

Immunogen: Thymocytes and T lymphocytes

Isotype: IgG2a

Clone: MEM-43

Description: CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor ofthe membrane attack complex (MAC). It is thus the key regulator that preserves theautologous cells from terminal effector mechanism of the complement cascade. CD59associates with C5b-8 complex and thereby counteracts appropriate formation of cytolyticpore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 andcauses T cell costimulation.

Specificity: The antibody MEM-43 reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on allhematopoietic cells, it is widely present on cells in all tissues.HLDA IV, WS Code NL 705HLDA V, WS Code AS S013HLDA V, WS Code BP BP345HLDA V, WS Code T T-103

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Cardiovascular, Atherosclerosis, Hematopoietic Progenitors, Tyrosine Kinases, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." in: Blood, Vol. 91, Issue 10, pp. 3901-8, 1998 (PubMed).