Salmonid Heat Shock Protein 70 (Hsp70) antibody

Antigen: Salmonid Heat Shock Protein 70 (Hsp70)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rainbow Trout (Oncorhynchus mykiss)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Catalog no.: ABIN125828

Additional Information

Immunogen: KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70.The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.

Format: Lyophilized

Description: Heat-shock protein 70 (Hsp70) is the major stress-inducible protein invertebrates and highly conserved throughout evolution. It plays a role as amolecular chaperone and is important for allowing cells to cope with acutestressor insult, especially those affecting the protein machinery.

Specificity: Predicted reactivity: atlantic salmon (Salmo salar), brook trout (Salvelinus fontinalis).

Application Details

Application Notes: Recommended Dilution 1:15 000 with standard ECL (WB), 5 µg of antibodies in reaction mixture (IP), 1:100 (IHC). Additional Information: immunohistochemistry experiments have been done on salmon tissue treated withhydrated autoclaving of formalin fixed sections (unpublished results) .

Buffer: Affinity purified serum in PBS pH 7.4

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Heat Shock Proteins

Restrictions: For Research Use only