KRT20 antibody

Catalog No. ABIN125990

The Mouse Monoclonal anti-KRT20 antibody is suitable to detect KRT20 in samples from Human and Pig. It has been validated for WB and IHC (fro).

Quick Overview for KRT20 antibody (ABIN125990)

Target: KRT20 (Keratin 20 (KRT20))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: KS20-5

Product Details anti-KRT20 Antibody

Specificity: IT-Ks 20.5 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20, Mr 46 000, formerly also designated cytokeratin 20). Reacts with human, pig, not with rat. Reacts with HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.

Purification: Protein A affinity chromatography

Immunogen: Electrophoretically purified keratin K20 from human intestinal mucosa.

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemical on frozen sections: 1: 10, 1h at RT or over night at 2-8C. Immunohistochemical on cytological material. Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL dist. water

Buffer: PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: Cytokeratin 20

Background: Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT

Gene ID: 54474

NCBI Accession: NP_061883

UniProt: P35900