YES1 antibody (N-Term)

Catalog No. ABIN126915

The Mouse Monoclonal anti-YES1 antibody (Clone 2B8) (ABIN126915) specifically detects YES1 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for YES1 antibody (N-Term) (ABIN126915)

Target: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This YES1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 2B8

Product Details anti-YES1 Antibody

Binding Specificity: N-Term

Specificity: AM00159PU-N specifically recognizes Yes at 59 kDa.

Characteristics: Synonyms: YES, p61-Yes, c-Yes, Proto-oncogene tyrosine-protein kinase Yes

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Use A431 untreated cell lysate as Positive Control. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Lyophilized Cell Lysate from serum starved A431 cellsReconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control is recommended for immunoblot applications. 20 µl of positive controlcell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation!

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Expiry Date: 12 months

Target Details for YES1

Target: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Alternative Name: YES1

Background: Yes is a member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Yes, src and fyn are ubiquitously expressed while the other members are primarily expressed in hematopoietic cells. Each protein tyrosine kinase (PTK) contains three domain (SH1, SH2, SH3). The SH1 domain has PTK activity, while the SH2 and SH3 domains are invloved in mediating protein-protein interactions by binding phosphotyrosine- containing and proline-riche motifs. By means of SH2-phosphotyrosine binding, PTKs are able to associate with activated receptor protein tyrosine kinases, e.g. platelet-derived growth factor (PDGFR)- and epidermal growth factor (EGF)- receptor.Synonyms: Proto-oncogene tyrosine-protein kinase Yes, YES, c-Yes, p61-Yes

Gene ID: 7525

UniProt: P07947

Pathways: CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling