FLIP antibody (C-Term, long form)

Catalog No. ABIN126994

This Rabbit Polyclonal antibody specifically detects FLIP in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FLIP antibody (C-Term, long form) (ABIN126994)

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FLIP Antibody

Binding Specificity: C-Term, long form

Specificity: The immunoaffinity-purified antibody detects 58 kDa FLIPL on SDS-PAGE immunoblots, in samples from human, mouse and rat origins. The antibody recognizes only the FLIPL form.

Purification: Affinity purified

Immunogen: Synthetic peptide near the C-terminus of human FLIPalpha/FLIPL form.

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thymerosal

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: CFLAR / Casper / I-FLICE

Background: Caspase-8 (FLICE) and -10 (FLICE2) are two pivotal members in the caspase family. Human and viral FLICE-inhibitory proteins were designated as v-FLIPs and c-FLIP, respectively. c-FLIP was cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH AND CLARP. c-FLIP contains two death effector domains and has sequence homology to caspase-8. c-FLIP interacts with adapter protein FADD and caspaser-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Two splice variants of c-FLIP have been identified and termed FLIPL and FLIPs. The mRNA for c-FLIP is predominantly expressed in muscle and lymphoid tissue.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP

Gene ID: 8837

NCBI Accession: NP_001120655

UniProt: O15519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development