PARP1 antibody (Asp214, C-Term, cleaved)

Catalog No. ABIN127025

The Rabbit Polyclonal anti-PARP1 antibody is suitable to detect PARP1 in samples from Mouse. It has been validated for WB, IF and IHC (p).

Quick Overview for PARP1 antibody (Asp214, C-Term, cleaved) (ABIN127025)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PARP1 Antibody

Binding Specificity: Asp214, C-Term, cleaved

Specificity: The polyclonal anti-cleaved PARP antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to the C-terminus adjacent to Asp214 of mouse PARP

Application Details

Application Notes: Western blot: 1-2 μg/mL. Immunocytochemistry: 10-20 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 50 % glycerol, 1 % BSA, and 0.02 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1

Background: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavege occurs between ASP214 and Gly 215, which separates PARP's N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.Synonyms: ADPRT, ADPRT 1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PPOL, Poly [ADP-ribose] polymerase 1, Poly[ADP-ribose] synthetase 1

Gene ID: 142

NCBI Accession: NP_001609

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location