GNAI2 antibody

Catalog No. ABIN127055

The Rabbit Polyclonal anti-GNAI2 antibody is suitable to detect GNAI2 in samples from Human. It has been validated for WB and IP.

Quick Overview for GNAI2 antibody (ABIN127055)

Target: GNAI2 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 2 (GNAI2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAI2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-GNAI2 Antibody

Specificity: The antibody detects human Gαi2. It also cross-reacts with Gαi3 and partially with Gαi1.

Purification: Affinity chromatography

Application Details

Application Notes: Western blot (0.5-1 μg/mL). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid Repeated freeze/defrost cycles.

Storage: -20 °C

Storage Comment: Store at -20 °C. For long term storage, aliquot and freeze at -70 °C.

Target Details for GNAI2

Target: GNAI2 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 2 (GNAI2))

Background: Gαi2 is a 40-41 kDa alpha-subunit of heterotrimeric protein, that shares sequence homology with Gαi3 and Gαi1. All these G proteins can be ADP-ribosylated at the carboxy terminal Cys of the CAAX sequence, interfering with receptor coupling. Gαi2 is expressed widely in tissues and cell lines. Activation of PLC-β by Gαi-coupled receptors is the result of release of Gβγ subunits from Gαi. Gαi2 regulates potassium channel activation. Mutation at Arg179 has been identified in tumors. This and the Q205L mutant inhibit adenylyl cyclase.Synonyms: Adenylate cyclase-inhibiting G alpha protein, GNAI2, GNAI2B, Guanine nucleotide-binding protein G(i), alpha-2 subunit

Gene ID: 2771

NCBI Accession: NP_002061

UniProt: P04899

Pathways: cAMP Metabolic Process, G-protein mediated Events, Thromboxane A2 Receptor Signaling