PKC beta antibody (C-Term)

Catalog No. ABIN127129

This Rabbit Polyclonal antibody specifically detects PKC beta in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow and Pig.

Quick Overview for PKC beta antibody (C-Term) (ABIN127129)

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC beta antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PKC beta Antibody

Binding Specificity: C-Term

Specificity: The antibody recognizes 60-75 kDa PKC beta isoforms from samples of human, mouse, rat, pig and bovine origins. A 40 kDa band can also be detected, presumably to be a shorter form of PKC isoforms.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to the C-terminus of human PKC.

Application Details

Application Notes: Western Blot: 1-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 50 % glycerol, 1 % BSA, and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for PKC beta

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Alternative Name: PRKCB

Background: PKC (Protein kinase C) is a calcium-dependent and phospholipid-dependent enzyme that is activated in vivo by the lipid diacylglycerol. PKC has been shown to be involved in signal transduction and various aspects of neoplastic transformation. PKC contains an amino terminal regulatory domain and a carboxy terminal catalytic domain joined by a hinge region. Cleavage of protein kinase C in vitro results in the generation of two functional fragments corresponding to these two domains.Synonyms: PKC beta, PKC-B, PKC-beta, PKCB, PRKCB1, Protein kinase C beta type

Gene ID: 5579

NCBI Accession: NP_002729

UniProt: P05771

Pathways: WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling