HDAC3 antibody (C-Term)

Catalog No. ABIN127236

The Rabbit Polyclonal anti-HDAC3 antibody (ABIN127236) specifically detects HDAC3 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HDAC3 antibody (C-Term) (ABIN127236)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HDAC3 Antibody

Binding Specificity: C-Term

Specificity: The antibody detects the 49 kDa HDAC3. It does not cross-react with other HDAC proteins including HDAC1, 2, 4, 5, 6, 7, and 8.

Purification: Peptide affinity purified.

Immunogen: Synthetic peptide corresponding to the C-terminus of human HDAC-3.

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. Immunohistochemistry: 20-30 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background: HDAC3 is a member of the class I mammalian histone deacetylase family, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC3 is composed of 428 amino acid residues. HDAC3 interacts with a growing number of transcriptional factors including SMRT and N-CoR. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.Synonyms: HD3, Histone deacetylase 3, RPD3-2, SMAP45

Gene ID: 8841

NCBI Accession: NP_003874

UniProt: O15379

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development