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Antibodies
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anti-Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) antibody
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) antibody
| Antigen |
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| Synonyms |
HME, MME, MGC138506, Mmel, AV378681, Mme, MMP12 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Flow Cytometry (FACS) (19), Western Blotting (WB) (19), Immunofluorescence (IF) (18), ELISA (8), Immunoprecipitation (IP) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (IHC) (5), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN127325 |
| Quantity |
0.1 mg (0.5 mg/ml) (Variants) |
| Price |
300.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Macrophage metalloelastase, HME, ME, Matrix metalloproteinase-12, MMP12, Macrophageelastase
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Alternative name
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MMP-12
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Swiss-Prot
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P39900
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Immunogen
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Synthetic peptide surrounding amino acid 460 of Human MMP-12
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Description
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The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible forthe degradation of extracellular matrix components, including collagen, gelatin,fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentiallyactivated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 may contribute to elastindegradation occurring in granulomatous skin diseases and may also participate inmacrophage migration through the epidermal and vascular basement membranes ininflammatory disorders.
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Specificity
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The antibody recognizes the MMP-12. Species: Human and Mouse. Others not tested. Add. Information: Blocking peptide (ABIN127324) is available separately.
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Application Notes
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Western Blot: 0.5-4.0 µg/ml. Mouse small intestine tissue lysate can be used as a Positive Control. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.5 mg/ml
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Buffer
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PBS containing 30% Glycerol and 0.02% Thimerosal.
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Storage
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Store the antibody undiluted at -20°C or for long term storage (in aliquots) at -70°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use only
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Alternatives for antigen "Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12)", type "Antibodies"
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Hosts
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Rabbit (36), Goat (2), Mouse (2)
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Reactivities
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Human (38), Mouse (Murine) (22), Rat (Rattus) (20), Dog (Canine) (3), Chicken (2), Cow (Bovine) (2), Horse (Equine) (2), Pig (Porcine) (2), Rabbit (2), Guinea Pig (1)
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Applications
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Flow Cytometry (FACS) (19), Western Blotting (WB) (19), Immunofluorescence (IF) (18), ELISA (8), Immunoprecipitation (IP) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (IHC) (5), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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HRP (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (3), Cleaved-Glu106 (1)
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Alternatives 
