MMP12 antibody (C-Term)

Catalog No. ABIN390136

This Rabbit Polyclonal antibody specifically detects MMP12 in WB, IHC (p) and IF. It exhibits reactivity toward Human and Mouse. It has been mentioned in 3+ publications

Quick Overview for MMP12 antibody (C-Term) (ABIN390136)

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: RB2026

Product Details anti-MMP12 Antibody

Binding Specificity: AA 391-420, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:2000. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-MMP12 antibody (ABIN390136)

Du, Wang, Tan, Li, Huang, Huang, Yang, Zhang, Wang, Huang, Huang: "Nkx2-5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E-Deficient Mice." in: Journal of the American Heart Association, Vol. 5, Issue 12, (2017) (PubMed).

LaPan, Brady, Grierson, Fleming, Miller, Sypek, Fu: "Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum." in: BMC pulmonary medicine, Vol. 10, pp. 40, (2010) (PubMed).

Ma, Flanc, Tesch, Bennett, Friedman, Nikolic-Paterson: "Blockade of the c-Jun amino terminal kinase prevents crescent formation and halts established anti-GBM glomerulonephritis in the rat." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 89, Issue 4, pp. 470-84, (2009) (PubMed).

Target Details for MMP12

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Alternative Name: MMP12

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP12 may be involved in tissue injury and remodeling. This protein has significant elastolytic activity. MMP12 can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, wtih small hydrophobic residues (prefereably alanine) occupying P3. The protein is found in alveolar macrophages but not in peripheral blood monocytes. MMP12 can be induced by exposure to lypopolysaccharide, and is inhibited by dexamethasone.

Molecular Weight: 54002

Gene ID: 4321

NCBI Accession: NP_002417

UniProt: P39900