Cyclin-Dependent Kinase 1 (CDK1) antibody

Antigen: Cyclin-Dependent Kinase 1 (CDK1)

Synonyms: CDK1, CDC28A, MGC111195, DKFZp686L20222, Cdc2, Cdc2a, p34, cdc2, MGC92032, zgc:92032, wu:fc30e01, CDC2, MGC134454, cdc-2, xcdc2, cdc2-a, cdc28a, p34cdc2, MGC53829

Clonality: Monoclonal (POH-1)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129504

Additional Information

Alternative name: cdc2 (p34)

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma P3- X63/AG8.653 cells using conventional hybridoma technology.

Format: Liquid (sterile filtered)

Isotype: IgG2a kappa  (Matching secondary antibodies)

Clone: POH-1

Description: P34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF). Tthe inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues. In humans the phosphorylation appears to performed by p60 src . The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins. The phosphorylation activity is coupled to the entry into the M-phase of the cell. p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally. Association with deletion mutants of cyclin halts the M-phase before it is completed.

Specificity: This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases. Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur. This reagent has broad interspecies reactivity.

Application Details

Application Notes: Recommended Dilutions: This product was assayed by immunoblot using HRP Goat-anti-Mouse IgG and ECL for detection. A working dilution of 1:500 to 1:1,000 is suggested for this assay. For immunohistochemistry dilute the antibody 1:50. Researchers should determine optimal titers for other applications. Note: This antibody is suitable for immunohistochemistry immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1% saponin treatment or other permeabilization method.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Research Area: CD Antigens, Surface Receptors of Immune Cells, Cell Cycle

Restrictions: For Research Use only

Images

Mab anti-Human p34cdc2 antibody (clone POH- 1) is shown to detect human p34cdc2by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human p34cdc2at room temperature for 30 min followed by detection using IRDye

Mab anti-Human p34cdc2 antibody was used to detect human p34cdc2by western blot in untreated (control) and drug treated (10 uM genistein) lysates of MCF-7 cells. Very strong detection occurs using a 1:1,000 dilution. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.