MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) antibody

Antigen: MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1)

Synonyms: MAD2, HSMAD2, AA673185, MGC113763, MAD2L1, cb408, zgc:112133, mad2, XMad2

Clonality: Polyclonal

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129510

Additional Information

Alternative name: MAD2L1

Gene ID: NP_002349

Swiss-Prot: Q13257

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with full-lengthrecombinant human MAD2L1 protein.

Format: Liquid (sterile filtered)

Isotype: IgG1  (Matching secondary antibodies)

Description: MAD2L1 (also called mitotic spindleassembly checkpoint protein, MAD2A, MAD2-like 1 andHsMAD2) is a component of the mitotic spindle assemblycheckpoint monitors the process of kinetochore-spindleattachment and delays the onset of anaphase when thisprocess is not complete. MAD2L1 inhibits the activity ofthe anaphase-promoting complex by sequesteringCDC20 until all chromosomes are aligned at themetaphase plate. MAD2L1 is related to the MAD2L2gene located on chromosome 1. This protein has anuclear localization. A MAD2 pseudogene has beenmapped to chromosome 14

Specificity: This Protein A purified antibody is directed against human MAD2L1 protein. The product was purifiedfrom tissue culture supernatant by chromatography. This antibody has only been tested on human cells. Reactivity againsthomologues from other sources is not known.

Application Details

Application Notes: This protein A purified antibodyhas been tested for use in immunoprecipitation and bywestern blot. This antibody is not applicable forimmunofluorescence microscopy. Specific conditions forreactivity should be optimized by the end user. Expect apredominant band at ~ 24 kDa corresponding to full-length protein by western blotting in the appropriate celllysate or extract. Dilutions: WESTERN BLOT 1:200 - 1:2,000 IMMUNOPRECIPITATION User Optimized IF MICROSCOPY Not Recommended OTHER APPLICATIONS User Optimized

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date ofopening.

Research Area: Cell Cycle

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Mab anti-MAD2L1 antibody shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 ug of lysate was separated by 4-20% TG SDSPAGE. After blocking, the membrane was probed overnight at 4