PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) antibody

Antigen: PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2)

Synonyms: PMSL2, HNPCC4, PMS2CL

Clonality: Monoclonal (349-29-5-2)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129513

Additional Information

Alternative name: PMS2

Gene ID: NP_000526

Swiss-Prot: P54278

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human PMS2corresponding to the first 133 amino acid residues of the protein. The clone was produced using conventional hybridoma technology. Hybridoma: Produced by the fusion between mouse splenocytes and mouse myeloma P3X63Ag8.653 cells using conventional hybridoma technology.

Format: Liquid (sterile filtered)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 349-29-5-2

Description: PMS2 is a highly conserved nuclear protein involved in mismatch repair during DNA replication and hasbeen identified to be composed as a heterodimer of PMS2 and MLH1. PMS is part of the BRCA1-associated genome surveillancecomplex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex.This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Defects in PMS2 arethe cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Turcot syndrome (an autosomal dominant disordercharacterized by malignant tumors of the brain associated with multiple colorectal adenomas) and supratentorial primitiveneuroectodermal tumors with cafe-au-lait spots (SNTCL). The human PMS2 gene encodes an 862 aa, 96 kDa polypeptide.

Specificity: This is an protein A purified antibody directed against PMS2-134 and reacts with full length version of PMS2in human and hamster tissues. The epitope was putatively mapped to amino acids 58-81 of human PMS2. BLAST analysis indicatesthat this sequence is 100% identical for human, mouse, rat and chimpanzee. No specific information is available for reactivity withPMS2 protein from other sources.

Application Details

Application Notes: This antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditionsfor western blotting reactivity should be optimized by the end user. Expect a band approximately 96 kDa in size corresponding tohuman PMS2 by western blotting in most cell lines and tissues as PMS2 is ubiquitously expressed. Dilutions: ELISA 1:5,000 - 1:20,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOPRECIPITATION User Optimized OTHER APPLICATIONS User Optimized

Concentration: 1.38 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.1% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage.Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product isstable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date ofopening.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis is shown using antibodies-online's Protein A Purified Mouse Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. Approximately 5,10 and 30 ug of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer. The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by washing. A 1:20,000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemical's SuperSignal