Nuclear Respiratory Factor 1 (NRF1) antibody

Antigen: Nuclear Respiratory Factor 1 (NRF1)

Synonyms: ALPHA-PAL, C87038, D6Ertd415e, ube2h, SC:BZ3C13.1, SC:bZ3C13.2, Nfe2l1_retired, IBR/F, NRF1, nrf-1, MGC83958, alpha-pal, LOC100220087

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129526

Additional Information

Gene ID: AAH05410

Swiss-Prot: Q9WU00

Immunogen: This protein A purified antibody was preparedfrom whole rabbit serum produced by repeated immunizationswith a purified recombinant mouse NRF1 proteincorresponding to aa 1- 534 of the native protein. Protein Sequence: Mouse NRF1, 534 aa, predicted MW 57.2 kDa 1 meehgvtqte hmatieahav aqqvqqvhva tytehsmlsa dedspssped tsyddsdiln 61 staadevtah laaagpvgma aaaavatgkk rkrphvfesn psirkrqqtr llrklratld 121 eyttrvgqqa ivlcispskp npvfkvfgaa plenvvrkyk smiledlesa laehapapqe 181 vnselpplti dgipvsvdkm tqaqlrafip emlkystgrg kpgwgkesck piwwpedipw 241 anvrsdvrte eqkqrvswtq alrtivkncy kqhgredlly afedqqtqtq attthsiahl 301 vpsqtvvqtf snpdgtvsli qvgtgatvat ladaselptt vtvaqvnysa vadgeveqnw 361 atlqggemti qttqaseatq avaslaeaav aasqemqqga tvtmalnsea aahavatlae 421 atlqgggqiv lsgetaaavg altgvqdang pdgvhawtis kavtapqaag kihtdfgkgf 481 imaevmkded lkeessenaa kaagkykqqg rayvvedgdi iffkfnspqq lkkk

Format: Liquid (sterile filtered)

Description: NRF1 (also known as nuclearrespiratory factor 1, alpha palindromic binding protein andalpha-pal) is the mammalian homolog to the erect wing(ewg) Drosophila protein that is required for properdevelopment of the central nervous system and indirectflight muscles. In mammals NRF1 functions as atranscription factor that activates the expression of theEIF2S1 (EIF2-alpha) gene. This protein links thetranscriptional modulation of key metabolic genes tocellular growth and development and has been implicatedin the control of nuclear genes required for respiration,heme biosynthesis, and mitochondrial DNA transcriptionand replication. NRF1 forms a homodimer and binds DNAas a dimer. NRF1 shows a nuclear localization and iswidely expressed in embryonic, fetal, and adult tissues.Phosphorylation of NRF1 enhances DNA binding. Multiplesplice variants have been identified for this protein.

Specificity: This protein A purified antibody is directed against mouse NRF1. The product was purified frommonospecific antiserum by protein A affinity purification. BLAST analysis was used to suggest reactivity with this protein frommouse, human, chimpanzee, dog, rat, chicken, frog and zebrafish based on very high sequence homology with theimmunogen sequence. Cross reactivity with NRF1 homologues from other sources has not been determined.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:3,000 - 1:10,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY 2 ug/ml - 5 ug/ml OTHER APPLICATIONS User Optimized. NoteThis protein A purified antibody has been tested for use in ELISA, immunohistochemistry and by westernblot. Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 67 kDa in sizecorresponding to NRF1 by western blotting in the appropriate celllysate or extract. Splice variants exist for this protein that mayresult in the detection of lower molecular weight bands.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Protein A Purified anti-NRF1 antibody shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye

Immunohistochemistry. antibodies-online's Affinity Purified anti-NRF1antibody was used at a 5 ug/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.