NRF1 antibody (Middle Region)

Catalog No. ABIN3044020

This Rabbit Polyclonal antibody specifically detects NRF1 in WB, IHC and ICC. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for NRF1 antibody (Middle Region) (ABIN3044020)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-NRF1 Antibody

Binding Specificity: AA 272-288, Middle Region

Purpose: Anti-Nuclear respiratory factor 1 NRF1 Antibody

Sequence: QHGREDLLYA FEDQQTQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Nuclear respiratory factor 1 NRF1 Antibody (ABIN3044020). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NRF1, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-NRF1 antibody (ABIN3044020)

Sun, Zhong, Zhang, Zhou: "Defect of mitochondrial respiratory chain is a mechanism of ROS overproduction in a rat model of alcoholic liver disease: role of zinc deficiency." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 310, Issue 3, pp. G205-14, (2016) (PubMed).

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: NRF1

Background:

Synonyms: Nuclear respiratory factor 1,NRF-1,Alpha palindromic-binding protein,Alpha-pal,NRF1,

Tissue Specificity: Ubiquitously expressed with strongest expression in skeletal muscle.

Background: Nuclear respiratory factor 1, is also known as NRF1. This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Sequence Similarities: Belongs to the NRF1/Ewg family.

Molecular Weight: 75 kDa

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha