NRF1 antibody (Middle Region)

Catalog No. ABIN3044020

Product Details anti-NRF1 Antibody

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Binding Specificity: AA 272-288, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for Nuclear respiratory factor 1(NRF1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequence: QHGREDLLYA FEDQQTQ

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Nuclear respiratory factor 1(NRF1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: nuclear respiratory factor 1
Protein Name: Nuclear respiratory factor 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NRF1(272-288aa QHGREDLLYAFEDQQTQ), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for NRF1 is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-NRF1 antibody (ABIN3044020)

Sun, Zhong, Zhang, Zhou: "Defect of mitochondrial respiratory chain is a mechanism of ROS overproduction in a rat model of alcoholic liver disease: role of zinc deficiency." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 310, Issue 3, pp. G205-14, (2016) (PubMed).

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: NRF1 (NRF1 Products)

Synonyms: ALPHA-PAL antibody, C87038 antibody, D6Ertd415e antibody, SC:BZ3C13.1 antibody, SC:bZ3C13.2 antibody, ube2h antibody, Nfe2l1_retired antibody, IBR/F antibody, NRF1 antibody, alpha-pal antibody, nrf-1 antibody, LOC100220087 antibody, nuclear respiratory factor 1 antibody, nuclear respiratory factor 1 S homeolog antibody, NRF1 antibody, Nrf1 antibody, nrf1 antibody, nrf1.S antibody

Background: NRF1(Nuclear Respiratory Factor 1), also known as Alpha-Pal. Gopalakrishnan and Scarpulla(1995) analyzed DNA from a panel of human/hamster cell hybrids using human-specific NRF1 PCR primers and localized the NRF1 gene to human chromosome 7. The assignment was further refined to 7q31 by cohybridization of NRF1- and chromosome 7-specific probes to human metaphase chromosomes. Efiok et al.(1994) identified genes containing alpha-Pal-binding sequences and found that these could be classified either as cellular proliferation genes, or as genes regulating the growth-responsive metabolic pathways of energy transduction, translation, and replication. Virbasius and Scarpulla(1994) noted that the nuclear-encoded mitochondrial transcription factor TFAM contains potential binding sites for NRF1, NRF2(GABPA) and SP1 within the promoter region. With use of binding and electrophoretic mobility shift assays, DNase footprinting, and mutation analysis of recombinant proteins, they demonstrated specific and functional binding of NRF1 and NRF2 to the TFAM promoter region.

Synonyms: alpha pal antibody|alpha palindromic binding protein antibody|Alpha palindromic-binding protein antibody|Alpha-pal antibody|locus control region factor 1 antibody|NFE2 related factor 1 antibody|NRF-1 antibody|Nrf1 antibody|NRF1_HUMAN antibody|nuclear factor(erythroid derived 2)-like 1 antibody|nuclear respiratory factor 1 antibody|transcription factor 11 antibody

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha