Ring Finger and FYVE-Like Domain Containing 1 (RFFL) antibody

Antigen: Ring Finger and FYVE-Like Domain Containing 1 (RFFL)

Synonyms: CARP2, RNF189, RNF34L, RIFIFYLIN, MGC84042, RFFL, Rffl

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129535

Additional Information

Alternative name: RFFL (Rififylin or RING finger and FYVE-Like Domain-Containing Protein 1)

Gene ID: NP_476519

Swiss-Prot: Q8WZ73

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-363 of human RFFL protein. Protein Sequence: Human RFFL, 363 aa, predicted MW 40.5 kDa 1 mwatccnwfc ldgqpeevpp pqgarmqays npgyssfpsp tglepscksc gahfantark 61 qtcldckknf cmtcssqvgn gprlcllcqr fratafqree lmkmkvkdlr dylslhdist 121 emcrekeelv llvlgqqpvi sqedrtrast lspdfpeqqa fltqphssmv pptspnlpss 181 saqatsvppa qvqenqqang hvsqdqeepv ylesvarvpa edetqsidse dsfvpgrras 241 lsdltdledi egltvrqlke ilarnfvnyk gccekwelme rvtrlykdqk glqhlvsgae 301 dqnggavpsg leenlckicm dspidcvlle cghmvtctkc gkrmnecpic rqyviravhv 361 frs

Format: Liquid (sterile filtered)

Description: RFFL, also known as Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like, is a novel modulator of NF- kappa B activation. RFFL possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RFFL also possesses anti-apoptotic activity and may bind phosphatidylinositol phosphates. RFFL is a membrane bound cytoplasmic protein that is expressed ubiquitously. RFFL can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes and is rapidly degraded after stimulation with TNFSF10, and probably by caspases. Multiple transcript variants have been detected for this protein.

Specificity: This protein A purified antibody is directed against human RFFL protein. The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.

Application Details

Application Notes: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RFFL protein by western blotting in the appropriate tissue or cell lysate or extract. Isoforms 2 and 3 are 39.7 and 36.6 kDa, respectively and should also cross react with this antibody. Dilution: ELISA 1:4,000 - 1:20,000 WESTERN BLOT 1:500 - 1:3,000 IMMUNOHISTOCHEMISTRY 1:500 - 1:3,000 OTHER APPLICATIONSS User Optimized

Concentration: 5.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 2° to 8° C prior to restoration. Restore with 0.1 ml of deionized water or equivalent. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Protein A Purified anti-RFFL antibody shows detection of RFFL (arrowhead) in lysate. Lanes correspond to empty vector 293T cell lysate (mock, left)

RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 uL of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RFFL. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000 using 5% BLOTTO, 0.1% Tween-20 in PBS as the diluent. Incubation occurred for 1 h at room temperature. Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.