RFFL antibody (Middle Region)

Catalog No. ABIN2775666

Cited in 1 publication. The Rabbit Polyclonal anti-RFFL antibody (ABIN2775666) specifically detects RFFL in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Horse, Dog, Rabbit and Guinea Pig samples.

Quick Overview for RFFL antibody (Middle Region) (ABIN2775666)

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFFL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RFFL Antibody

Binding Specificity: Middle Region

Purpose: RFFL Antibody - middle region

Sequence: KDQKGLQHLV SGAEDQNGGA VPSGLEENLC KICMDSPIDC VLLECGHMVT

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RFFL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RFFL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42986-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RFFL antibody (ABIN2775666)

Hanuszkiewicz, Pittock, Humphries, Moll, Rosales, Molinaro, Moynagh, Lajoie, Valvano: "Identification of the flagellin glycosylation system in Burkholderia cenocepacia and the contribution of glycosylated flagellin to evasion of human innate immune responses." in: The Journal of biological chemistry, Vol. 289, Issue 27, pp. 19231-44, (2014) (PubMed).

Target Details for RFFL

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Alternative Name: RFFL

Background:

Background Information: RFFL has E3 ubiquitin protein ligase activity.RFFL regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity.RFFL may bind phosphatidylinositol phosphates.

Gene Name: Ring finger and FYVE-like domain containing E3 ubiquitin protein ligase

Alternative Symbols: CARP2, FRING, CARP-2, RNF189, RNF34L, RIFIFYLIN

Protein Name: E3 ubiquitin-protein ligase rififylin

Molecular Weight: 40kDa

Gene ID: 117584

NCBI Accession: NP_001017368

UniProt: Q8WZ73