Ring Finger Protein 34 (RNF34) antibody

Antigen: Ring Finger Protein 34 (RNF34)

Synonyms: RFI, RIF, RIFF, CARP1, FLJ21786, MGC134085, RNF34, DKFZp459C1115, rnf34

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129536

Additional Information

Alternative name: RNF34 (RING finger protein 34)

Gene ID: NP_919247

Swiss-Prot: Q969K3

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-373 of human RNF34 protein.

Format: Liquid (sterile filtered)

Description: RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF- kappa B activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RNF34 protects cells against apoptosis induced by TNF and may bind phosphatidylinositol phosphates. RNF34 is localized to both the nucleus and the cytoplasm. In the nucleus, a distinct speckled pattern is observed by immunofluorescence microscopy. In the cytoplasm, the protein is associated with membranes. RNF34 is expressed ubiquitously and can be detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Multiple transcript variants have been reported for this protein.

Specificity: This protein A purified antibody is directed against human RNF34 protein. The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above. A BLAST analysis was used to suggest cross-reactivity with RNF34 protein from orangutan (99%), bovine (91%), dog (89%), mouse (88%) and rat (85%) based on a high degree of protein:protein homology with the immunizing sequence. Expect reactivity with isoform 1 and 2 of RNF34. Reactivity against homologues from other sources is not known. ELISA 1:4,000 - 1:20,000 WESTERN BLOT 1:500 - 1:3,000 IF MICROSCOPY User Optimized OTHER APPLICATIO User Optimized

Application Details

Application Notes: This protein A purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 42 kDa in size corresponding to RNF34 protein by western blotting in the appropriate tissue or cell lysate or extract. Isoforms 1 and 2, which are equivalent in size, should both cross-react with this antibody.

Concentration: 5.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 2° to 8° C prior to restoration. Restore with 0.1 ml of deionized water or equivalent. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Research Area: Ion Channels

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Protein A Purified anti-RNF34 antibody shows detection of human RNF34 (arrowhead) in lysate. Lanes corresponding to empty vector 293T cell lysate (mock, left)