AGAP2 antibody (AA 1-836)

Catalog No. ABIN129537

Supplier Product No.: 200-401-946

The Rabbit Polyclonal anti-AGAP2 antibody (ABIN129537) specifically detects AGAP2 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for AGAP2 antibody (AA 1-836) (ABIN129537)

Target: AGAP2 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-AGAP2 Antibody

Binding Specificity: AA 1-836

Supplier Product No.: 200-401-946

Supplier: Rockland

Purpose: AGAP2 Antibody

Cross-Reactivity (Details): This protein A purified antibody is directed against human AGAP2 protein.

Characteristics: Synonyms: rabbit anti-AGAP2 antibody, AGAP-2, AGAP 2, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 antibody, ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody, centaurin gamma1 antibody, Centg1 antibody

Purification: The product was purified from monospecific antiserum by protein A chromatography.

Immunogen:

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-836 of human AGAP2 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein A purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to AGAP2 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:5,000 - 1:20,000

ELISA Dilution: 1:40,000 - 1:200,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for AGAP2

Target: AGAP2 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2))

Alternative Name: AGAP2

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). AGAP2 (also known as Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2) is a GTPase activating protein that inactivates Arf. The expression of AGAP2 is amplified in human glioblastoma cells. GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. Anti-AGAP2 Antibody is useful for researchers interested in Developmental Biology, apoptosis, Cancer, Immunology, and Nuclear Signaling research.

Gene ID: 116986, 7661962

UniProt: Q99490