AGAP2 antibody (Internal Region)

Catalog No. ABIN570975

The Goat Polyclonal anti-AGAP2 antibody (ABIN570975) specifically detects AGAP2 in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for AGAP2 antibody (Internal Region) (ABIN570975)

Target: AGAP2 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AGAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-AGAP2 Antibody

Binding Specificity: Internal Region

Purpose: CENTG1 / PIKE

Sequence: QASLDSIREA VINSQ

Specificity: This antibody is expected to recognize isoform PIKE-S (NP_055585.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QASLDSIREAVINSQ, from the internal region of the protein sequence according to NP_055585.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thyroid and Kidney. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 100 kDa band observed in Human Brain (Amygdala and Cerebral Cortex) lysates (calculated MW of 90.5 kDa according to NP_055585.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AGAP2

Target: AGAP2 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2))

Alternative Name: AGAP2

Background: CENTG1, centaurin, gamma 1, AGAP2, FLJ16430, GGAP2, KIAA0167, PIKE, Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2, GTP-binding and GTPase activating protein 2, centaurin gamma1, phosphoinositide 3-kinase enhancer

Gene ID: 116986

NCBI Accession: NP_055585