MTBP antibody

Antigen: MTBP

Clonality: Monoclonal (PHL-1)

Host: Mouse

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129549

Additional Information

Gene ID: NP_071328

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with arecombinant amino-terminal HIS 6X -tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein. Hybridoma: Produced by the fusion between mouse splenocytes and mouse myeloma P3X63Ag8.653 cells using conventional hybridoma technology.

Format: Liquid (sterile filtered)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: PHL-1

Description: The MDM2 onco-protein is overexpressed in human cancer cells and itpromotes tumorigenesis by directly blocking thefunction of the p53 tumor suppressor. Studiesalso indicate that MDM2 regulates cell prolifer-ation via p53 independent mechanisms. MTBP(Mdm2 [Two] Binding Protein) was identified bya yeast two-hybrid screen as an MDM2 bindingprotein (1). MTBP has been reported tomodulate cell cycle progression by a p53independent mechanism and suppress MDM2-mediated p53 degradation when ectopicallyexpressed in cells (1,2). The human MTBPgene localizes to 8q24 (3) and MTBP RNA isexpressed in a wide range of tissues, withhighest being in the thymus, testes and ovaries(1).

Specificity: This antibody is directed against human MTBP protein. The product was producedfrom clarified mouse ascetic fluid. A BLAST analysis was used to suggest reactivity with this protein fromhuman (100% homology) and chimpanzee. Cross reactivity with MTBP protein from mouse (77% homology)and chicken (79% homology) is unlikely due to the relatively low number of positive residues and high numberof gaps within this sequence. Cross reactivity with MTBP homologues from other sources has not beendetermined. ELISA 1:2,000 - 1:20,000 WESTERN BLOT 1:200 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Application Details

Application Notes: Recommended Dilutions: ELISA 1:2,000 - 1:20,000 WESTERN BLOT 1:200 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 110 kDa in size corresponding to MTBP by western blotting in the appropriate cell lysate or extract. Dilutions: Protein Sequence: Human MTBP, 904 aa 1 mdrylllviw gegkfpsaas reaehgpevs sgegtenqpd ftaanvyhll krsisasinp 61 edstfpacsv ggipgskkwf favqaiygfy qfcssdwqei hfdtekdkie dvlqtnieec 121 lgavecfeee dsnsreslsl adlyeeaaen lhqlsdklpa pgramvdiil llsdkdppkl 181 kdylptvgal khlrewysak itiagnhcei ncqkiaeyls anvvsledlr nvidskelwr 241 gkiqiwerkf gfeisfpefc lkgvtlknfs tsnlntdfla kkiipskdkn ilpkvfhyyg 301 palefvqmik lsdlpscyms diefelgltn stkqnsvlll eqisslcskv galfvlpcti 361 snilipppnq lssrkwkeyi akkpktisvp dvevkgecss yylllqgngn rrckatlihs 421 anqingsfal nlihgkmktk teeaklsfpf dllslphfsg eqivqrekql anvqvlalee 481 clkrrklakq petvsvaelk sllvltrkhf ldyfdavipk milrkmdkik tfnilndfsp 541 vepnssslme tnplewperh vlqnletfek tkqkmrtgsl phsseqllgh kegprdsitl 601 ldakellkyf tsdglpigdl qplpiqkgek tfvltpelsp gklqvlpfek asvchyhgie 661 yclddr kale rdggfselqs rliryetqtt ctresfpvpt vlsplpspvv ssdpgsvpdg 721 evlqnelrte vsrlkrrskd lnclyprkrl vksessesll sqttgnsnhy hhhvtsrkpq 781 terslpvtcp lvpipscetp klatktssgq ks mhesktsr qikesrsqkh trilkevvte 841 tlkkhsitet hecftacsqr lfeiskfylk dlktsrglfe emkktannna vqvidwvlek 901 tskk

Concentration: Not determined

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extendedstorage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration dateis one (1) year from date of opening.

Research Area: Cancer, Cell Cycle

Restrictions: For Research Use only

Images

Western blot using antibodies-online's anti-MTBP antibody shows detection of a band ~110 kDa corresponding to human MTBP (arrowhead). Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct. The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP. Proteins were separated by SDS-PAGE and transferred onto PDVF membrane. After blocking, the membrane was probed with the primary antibody diluted to 1:500 for 2h at room temperature followed by detection using a Lumi-LightPlus Western Blotting Kit (Roche).