MTBP antibody (Middle Region)

Catalog No. ABIN500294

The Rabbit Polyclonal anti-MTBP antibody is suitable to detect MTBP in samples from Human. It has been validated for WB, EIA and IF.

Quick Overview for MTBP antibody (Middle Region) (ABIN500294)

Target: MTBP (MDM2 Binding Protein (MTBP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTBP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Product Details anti-MTBP Antibody

Binding Specificity: AA 122-139, Middle Region

Specificity: This antibody detects MTBP at center.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Peptide affinity chromatography

Immunogen: MTBP antibody was raised against a synthetic peptide corresponding to amino acids 122 to 139 of human MTBP, which differ from the mouse sequence by three amino acids (7).

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for MTBP

Target: MTBP (MDM2 Binding Protein (MTBP))

Alternative Name: MTBP

Background: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6) and MTBP (7), were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53 (7). MTBP is expressed in a variety of normal tissues (7).Synonyms: Mdm2, Mdm2-binding protein, hMTBP

Gene ID: 27085

NCBI Accession: NP_071328

UniProt: Q96DY7