Polymerase (DNA Directed) kappa (POLK) antibody

Antigen: Polymerase (DNA Directed) kappa (POLK)

Synonyms: DINP, POLQ, DINB1, Dinb1, POLK, polk, MGC88914, MGC152198, DKFZp459J211, si:ch211-254o18.3

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129559

Additional Information

Alternative name: POLK (polymerase (DNA directed) polymerase kappa)

Immunogen: This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizationswith a synthetic peptide corresponding to amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa). Protein Sequence: Human POLK (isoform 1), 870 aa, predicted MW 98.8 kDa 1 mdstkekcds ykddlllrmg lndnkagmeg ldkekinkii meatkgsrfy gnelkkekqv 61 nqrienmmqq kaqitsqqlr kaqlqvdrfa meleqsrnls ntivhidmda fyaavemrdn 121 pelkdkpiav gsmsmlstsn yharrfgvra ampgfiakrl cpqliivppn fdkyravske 181 vkeiladydp nfmamsldea ylnitkhlee rqnwpedkrr yfikmgssve ndnpgkevnk 241 lsehersisp llfeespsdv qppgdpfqvn feeqnnpqil qnsvvfgtsa qevvkeirfr 301 ieqkttltas agiapntmla kvcsdknkpn gqyqilpnrq avmdfikdlp irkvsgigkv 361 tekmlkalgi itctelyqqr allsllfset swhyflhisl glgsthltrd gerksmsver 421 tfseinkaee qyslcqelcs elaqdlqker lkgrtvtikl knvnfevktr astvssvvst 481 aeeifaiake llkteidadf phplrlrlmg vrissfpnee drkhqqrsii gflqagnqal 541 satectlekt dkdkfvkple mshkksffdk krserkwshq dtfkceavnk qsfqtsqpfq 601 vlkkkmnenl eisensddcq iltcpvcfra qgcislealn khvdecldgp sisenfkmfs 661 cshvsatkvn kkenvpassl cekqdyeahp kikeissvdc ialvdtidns skaesidals 721 nkhskeecss lpsksfnieh chqnssstvs lenedvgsfr qeyrqpylce vktgqalvcp 781 vcnveqktsd ltlfnvhvdv clnksfiqel rkdkfnpvnq pkessrstgs ssgvqkavtr 841 tkrpglmtky stskkikpnn pkhtldiffk

Format: Liquid (sterile filtered)

Description: POLK (also known as DNA polymerase kappa,DINB protein and DINP) is involved in DNA repair. POLKplays an important role in translesion synthesis, where thenormal high-fidelity DNA polymerases cannot proceed andDNA synthesis stalls. Depending on the context, it inserts thecorrect base, but causes frequent base transitions,transversions and frameshifts. POLK lacks 3'-5' proofreadingexonuclease activity. POLK is a nuclear protein that binds toREV1L and PCNA. POLK is detected throughout the nucleusand at replication foci. Multiple alternative splice variants havebeen detected for this protein. POLK is found at low levels intestis, spleen, prostate and ovary and at very low levels inkidney, colon, brain, heart, liver, lung, placenta, pancreas andperipheral blood leukocytes.

Specificity: This affinity purified antibody is directed against human POLK protein. The product was affinitypurified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivitywith this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence.Cross reactivity with POLK protein from mouse is expected, as this sequence is highly homologous in human andmouse. Expect reactivity with multiple splice variants of this protein. Cross reactivity with POLK homologues from othersources has not been determined.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:3,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specificconditions for reactivity should be optimized by the end user. Expect a band ~ 98.8 kDa in size corresponding to POLKby western blotting in the appropriate cell lysate or extract. Splice variants for this protein may show molecular weightsof 89.0 and 76.4 kDa.

Concentration: 1.4 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening.Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cyclesof freezing and thawing. Expiration date is one (1) year fromdate of opening.

Research Area: Enzymes

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Affinity Purified anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead). Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDSPAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4