V5 Epitope Tag (GKPIPNPLLGLDST) antibody

Antigen: V5 Epitope Tag

Epitope: GKPIPNPLLGLDST

Clonality: Polyclonal

Host: Rabbit

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129577

Additional Information

Alternative name: V5 Epitope TAG Sequence: G-K-P-I-P-N-P-L-L-G-L-D-S-T

Gene ID: YP_138513

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with V5 epitope tagpeptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide. Protein Sequence: V protein [Simian parainfluenza virus 5], 222 aa, predicted MW 167.7 kDa 1 mdptdlsfsp deinklietg lntveyftsq qvtgtsslgk ntippgvtgl ltnaaeakiq 61 estnhqkgsv gggakpkkpr pkiaivpadd ktvpgkpipn pllgldstps tqtvldlsgk 121 tlpsgsykgv klakfgkenl mtrfieepre npiatsspid fkrgrdtggf hrreysigwv 181 gdevkvtewc npscspitaa arrfectchq cpvtcsecer dt

Format: Liquid (sterile filtered)

Description: Epitope tags are short peptidesequences that are easily recognized by tag-specificantibodies. Due to their small size, epitope tags do not affectthe tagged protein's biochemical properties. Most oftensequences encoding the epitope tag are included with targetDNA at the time of cloning to produce fusion proteinscontaining the epitope tag sequence. This allows anti-epitopetag antibodies to serve as universal detection reagents for anytag containing protein produced by recombinant means. Thismeans that anti-epitope tag antibodies are a useful alternativeto generating specific antibodies to identify, immunoprecipitateor immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety ofantibody-dependent experimental procedures. Expressionvectors producing epitope tag fusion proteins are available fora variety of host expression systems including bacteria, yeast,insect and mammalian cells. Antibodies-Online Immunochemicalsproduces anti-epitope tag antibodies against many commonepitope tags including Myc, GST, GFP, 6X His, MBP, FLAG,HA and V5. Antibodies-Online Immunochemicals also producesantibodies to other tags including FITC, Rhodamine (TRITC),DNP and biotin.

Specificity: This affinity-purified antibody is directed against V5 motif and is useful in determining its presence invarious assays. This polyclonal anti-V5-tag antibody detects over-expressed proteins containing the V5 epitope tag. To datethis antibody has reacted with all V5 tagged proteins tested so far. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. The antibody recognizes the V5-epitope tag (GKPIPNPLLGLDST) fused to eitherthe carboxy- terminal end of targeted proteins in transfected or transformed cells. Although not yet tested, expect reactivitywith recombinant proteins prepared with the V5-epitope tag fused to the amino terminal end as well.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:10,000 - 1:60,000 WESTERN BLOT 1:5,000 - 1:10,000 IMMUNOHISTOCHEMISTRY 1:500 - 1:3,000 OTHER APPLICATIONS User Optimized. Note: Anti-V5 is optimally suited for monitoring expression of V5-tagged fusion proteins. The V5 epitope tag isderived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag isusually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acidsequence (IPNPLLGLD). This antibody has been tested by ELISA and western blotting against both the immunizing peptideand V5 containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation andimmunocytochemistry.

Concentration: 1.54 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C. For extended storage aliquot contents and freeze at -20° C or below. Avoid cyclesof freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

Anti-V5 EPITOPE TAG polyclonal antibody detects V5-tagged recombinant protein by western blot. This antibody was used at 1.0 ug/ml to detect 0.05 ug (lane 2) of full-length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS-PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4