V5 Epitope Tag antibody

Catalog No. ABIN3029565

Product Details anti-V5 Epitope Tag Antibody

Target: V5 Epitope Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This V5 Epitope Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Sequence: GKPIPNPLLG LDST

Purification: Purified

Immunogen: The V5 sequence GKPIPNPLLGLDST was used as the immunogen for the V5 tag antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the V5 Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the V5 Tag antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

References for anti-V5 Epitope Tag antibody (ABIN3029565)

Aagaard, Fischer, Lober, Neumann, Allahverdi, Sivelle, Miehe, Spillner: "Extract-Shaped Immune Repertoires as Source for Nanobody-Based Human IgE in Grass Pollen Allergy." in: Molecular biotechnology, Vol. 65, Issue 9, pp. 1518-1527, (2023) (PubMed).

Target Details for V5 Epitope Tag

Target: V5 Epitope Tag

Alternative Name: V5 Tag (V5 Epitope Tag Products)

Target Type: Tag

Background: Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). NSJBio's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.