Red Fluorescent Protein (RFP) antibody

Antigen: Red Fluorescent Protein (RFP)

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Catalog no.: ABIN129578

Additional Information

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Format: Liquid (sterile filtered)

Description: Anti-RFP (RABBIT) Antibody Min X Hu Ms and Rt Serum Proteins

Specificity: Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.

Application Details

Application Notes: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. Recommended Dilutions: ELISA: 1:100,000. WB: 1:2,000 - 1:5,000. IHC: 1:200 - 1:1000.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Purification: This product was prepared from monospecific antiserum by imunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

anti-Red Fluorescent Protein (RFP) antibody

Publications

Publications: Shi, Wu, Liu et al.: "Chitosan fibers: versatile platform for nickel-mediated protein assembly." in: Biomacromolecules, Vol. 9, Issue 5, pp. 1417-23, 2008 (PubMed).

Dallaire, Bellmann, Laplante et al.: "Obese mice lacking inducible nitric oxide synthase are sensitized to the metabolic actions of peroxisome proliferator-activated receptor-gamma agonism." in: Diabetes, Vol. 57, Issue 8, pp. 1999-2011, 2008 (PubMed).

Burton, Johnson, Sato et al.: "Adenovirus-mediated gene expression imaging to directly detect sentinel lymph node metastasis of prostate cancer." in: Nature medicine, Vol. 14, Issue 8, pp. 882-8, 2008 (PubMed).

Becher, Hambardzumyan, Walker et al.: "Preclinical evaluation of radiation and perifosine in a genetically and histologically accurate model of brainstem glioma." in: Cancer research, Vol. 70, Issue 6, pp. 2548-57, 2010 (PubMed).

Fries, Reddy, Mikhaylova et al.: "Dynamic cellular translocation of caldendrin is facilitated by the Ca2+-myristoyl switch of recoverin." in: Journal of neurochemistry, Vol. 113, Issue 5, pp. 1150-62, 2010 (PubMed).

Chambers, Takimoto: "Trafficking of Sendai virus nucleocapsids is mediated by intracellular vesicles." in: PLoS ONE, Vol. 5, Issue 6, pp. e10994, 2010 (PubMed).