Polynucleotide Kinase 3'-Phosphatase (PNKP) antibody

Antigen: Polynucleotide Kinase 3'-Phosphatase (PNKP)

Synonyms: PNK, MGC94448, PNKP, Tb06.28P18.320, 21.m03011

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129595

Additional Information

Alternative name: PNK (Polynucleotide Kinase)

Gene ID: NP_009185

Swiss-Prot: Q96T60

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PNK protein.

Format: Liquid (sterile filtered)

Description: Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'-phosphatase and Polynucleotide kinase) catalyzes the 5' phosphorylation of nucleic acids and can have associated 3' phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.

Specificity: This affinity purified antibody is directed against human PNK protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Expect cross-reactivity with PNK from dog, mouse and rat sources, as only two amino acid residues change within the immunogen sequence (83% positive by BLAST). Cross-reactivity with PNK homologues from other sources has not been determined.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size corresponding to PNK by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:3,000 - 1:12,000 WESTERN BLOT 1:300 - 1:1,500 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 1.46 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Kinases/Phosphatases

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Affinity Purified anti-PNK antibody shows detection of a 57 kDa band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature. Signal was detected using standard techniques.