HUS1 Checkpoint Homolog B (S. Pombe) (HUS1B) antibody

Antigen: HUS1 Checkpoint Homolog B (S. Pombe) (HUS1B)

Synonyms: MGC126746, MGC126748, RP11-532F6.1, HUS1B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129605

Additional Information

Alternative name: Hus1B

Gene ID: NP_683762

Swiss-Prot: Q8NHY5

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 104-117 of human Hus1B protein. Protein Sequence: Human Hus1B, 278 aa, predicted MW 31.0 kDa 1 mkfrakitgk gclelfihvs gtvarlakvc vlrvrpdslc fgpagsgglh earlwcevrq 61 gafqqfrmeg vsedldeihl eltaehlsra arsaagassl klqlthkrrp sltvavelvs 121 slgrarsvvh dlpvrvlprr vwrdclppsl rasdasirlp rwrtlrsive rmanvgshvl 181 veanlsgrmt lsietevvsi qsyfknlgnp pqsavgvpen rdlesmvqvr vdnrkllqfl 241 egqqihptta lcniwdntll qlvlvqeyvs lqyfipal

Format: Liquid (sterile filtered)

Description: The protein encoded by theHus1B gene is most closely related to HUS1, acomponent of a cell cycle checkpoint protein complexinvolved in cell cycle arrest in response to DNA damage.This protein can interact with the checkpoint proteinRAD1 but not with RAD9. Over expression of this proteinhas been shown to induce cell death, which suggests arelated but distinct role of this protein, as compared toHUS1. Hus1B is expressed strongly in testis, less inspleen, thymus, prostate, colon and leukocytes.

Specificity: This affinity-purified antibody is directed against human Hus1B protein. The product wasaffinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis indicates 100%homology of the immunizing sequence with Hus1B protein from human. Partial reactivity with mouse Hus1B isnot likely based on low (~64%, 9/14) sequence homology. Reactivity against homologues from other sources isnot known.

Application Details

Application Notes: This affinity-purified antibody has been tested for use in ELISA and western blot. Specificconditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in sizecorresponding to Hus1B protein by western blotting in the appropriate cell lysate or extract.

Concentration: 1.75 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extendedstorage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date isone (1) year from date of opening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's affinity purified anti-Hus1B antibody shows detection of a 36kDa band corresponding to Hus1B in a HeLa cell lysate (arrowhead). The staining pattern in 293 cells is less clear, showing a predominant band at 39 kDa. Personal Communication, ALien Lu-Chang, U. Maryland, Baltimore, MD.