Werner Helicase Interacting Protein 1 (WRNIP1) antibody

Antigen: Werner Helicase Interacting Protein 1 (WRNIP1)

Synonyms: WRNIP1, wrnip1, MGC148461, MGC159472, WHIP, FLJ22526, bA420G6.2, RP11-420G6.2, Wrnip, 4833444L21Rik, Whip, MGC112594, MGC85976

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129611

Additional Information

Alternative name: WHIP

Gene ID: CAH73664

Swiss-Prot: Q96S55

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa.

Format: Liquid (sterile filtered)

Description: Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process. A second isoform exists (WHIP2).

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide immobilized to a solid phase. Reactivity is expected against human, mouse, rat and monkey WHIP1 protein. Cross reactivity with WHIP2 protein (isoform2) from human sources is expected based on high homology (BLAST). Reactivity with WHIP proteins from other sources is not known, but likely due to reported homologies.

Application Details

Application Notes: This affinity purified antibody has been tested for use in western blotting against HEK293 whole cell lysates. The antibody is also functional by ELISA. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 96.0 kDa (predicted molecular weight: 72.2 kDa). Specific band detection by western blot is blocked by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown. Dilutions: ELISA 1:10,000 - 1:40,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 1.10 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis is shown using antibodies-online's Affinity Purified anti-Human WHIP antibody to detect Human WHIP present in a HEK293 whole cell lysate. ~30ug of lysate was loaded per lane for 4-20% gradient SDSPAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye

Western blot analysis is shown using antibodies-online's anti-Human WHIP antibody with and without pre-incubation with blocking peptide. Testing was performed on antiserum prior to affinity purification. Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WHIP1. ~30ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 kDa is detected. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye