RAD54-Like (S. Cerevisiae) (RAD54L) antibody

Antigen: RAD54-Like (S. Cerevisiae) (RAD54L)

Synonyms: SHS, XH2, XNP, ATR2, SFM1, RAD54, MRXHF1, RAD54L, ZNF-HX, MGC2094, HR54, hHR54, RAD54A, hRAD54, MGC189414, MGC56289, zgc:56289, rad54l, MGC69368, GdRAD54

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129616

Additional Information

Alternative name: Rad54

Gene ID: NP_003570

Swiss-Prot: Q92698

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with asynthetic peptide corresponding aa 1-17 of Human RAD54 protein. Protein Sequence: Human RAD54 protein, 747 aa, predicted MW 84.4 kDa 1 mrrslapsql akrkpegrsc ddedwqpglv tprkrkssse tqiqecflsp frkplsqltn 61 qppcldssqh eafirsilsk pfkvpipnyq gplgsralgl kragvrralh dplekdalvl 121 yeppplsahd qlkldkeklp vhvvvdpils kvlrphqreg vkflwecvts rripgshgci 181 mademglgkt lqcitlmwtl lrqspeckpe idkavvvsps slvknwynev gkwlggriqp 241 laidggskde idqklegfmn qrgarvsspi liisyetfrl hvgvlqkgsv glvicdeghr 301 lknsenqtyq aldslntsrr vlisgtpiqn dlleyfslvh fvnsgilgta hefkkhfelp 361 ilkgrdaaas eadrqlgeer lreltsivnr clirrtsdil skylpvkieq vvccrltplq 421 telykrflrq akpaeelleg kmsvsslssi tslkklcnhp aliydkcvee edgfvgaldl 481 fppgysskal epqlsgkmlv ldyilavtrs rssdkvvlvs nytqtldlfe klcrarryly 541 vrldgtmsik krakvverfn spsspdfvfm lsskaggcgl nliganrlvm fdpdwnpand 601 eqamarvwrd gqkktcyiyr llsagtieek ifqrqshkka lsscvvdeeq dverhfslge 661 lkelfildea slsdthdrlh crrcvnsrqi rpppdgsdct sdlagwnhct dkwglrdevl 721 qaawdaasta itfvfhqrsh eeqrglr

Format: Liquid (sterile filtered)

Description: RAD54, also known as hHR54, HR54, hRAD54 and RAD54A, belongs to the DEAD-like helicasesuperfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologousrecombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNAdouble-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought tofacilitate homologous DNA pairing, and stimulate DNA recombination.

Specificity: This product is an affinity-purified antibody produced by immunoaffinity chromatography using peptidecoupled to agarose beads. BLAST analysis indicates 100 % homology of the immunizing sequence with RAD54 from human. Crossreactivity with RAD54 protein homologues from other sources may not occur as sequence homology varies by at least one amino acidresidue in this sequence.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions forreactivity should be optimized by the end user. Expect a band approximately 84 kDa in size corresponding to RAD54 protein bywestern blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lowermolecular weight bands. Dilutions: ELISA 1:10,000 - 1:40,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contentsand freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Model for generation of supercoiling by hRad54 translocation along DNA. The hRad54 complex and plasmid DNA are indicated by the shaded oval and black line, respectively. (A) Movement of the hRad54 complex by tracking along the helical path of DNA is indicated by the arrows. When the complex is free to rotate around the DNA, no change in supercoiling will be induced in the plasmid DNA. (B) When the hRad54 complex tracks along the helix, while being prevented from rotating around the DNA, positive supercoils will arise ahead of the protein complex and negative supercoils behind it. These supercoils can freely distribute along the plasmid and therefore they will cancel each other out. (C) The interaction of two hRad54 complexes on a plasmid will divide the plasmid into two domains. Because the plasmid is singly nicked, one domain will contain a nick, whereas the other contains two covalently closed DNA strands. Depending on the position of the nick relative to the movement of the protein complex along the DNA, topoisomers containing either negative or positive supercoils will result after ligation of the nick.

anti-RAD54-Like (S. Cerevisiae) (RAD54L) antibody (Image 2)