SET Domain, Bifurcated 1 (SETDB1) antibody

Antigen: SET Domain, Bifurcated 1 (SETDB1)

Synonyms: ESET, KG1T, KMT1E, KIAA0067, H3-K9-HMTase4, AU022152, MGC90670, mKIAA0067, SETDB1, NV10069, fc07d03, wu:fc07d03, si:ch211-81a5.6

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129618

Additional Information

Alternative name: ESET

Gene ID: NP_036564

Swiss-Prot: Q15047

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with asynthetic peptide corresponding to amino acids 1058-1075 of human ESET.

Format: Liquid (sterile filtered)

Description: The SET domain is a highly conserved,approximately 150-amino acid motif implicated in the modulation ofchromatin structure. It was originally identified as part of a largerconserved region present in the Drosophila trithorax protein andwas subsequently identified in the Drosophila Su(var)3-9 and'Enhancer of zeste' proteins, from which the acronym SET isderived. Studies have suggested that the SET domain may be asignature of proteins that modulate transcriptionally active orrepressed chromatin states through chromatin remodelingactivities. ESET functions as a histone methyltransferase bymethylation of Lys-9 of histone H3. H3 Lys-9 methylationrepresents a specific tag for epigenetic transcriptional repression byrecruiting HP1 proteins to methylated histones. ESET shows anuclear localization and is associated with non-pericentromericregions of chromatin, and is excluded from nucleoli and islands ofcondensed chromatin. Although ESET is a widely expressedprotein, it is highly expressed in the testis.

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using theimmunizing peptide after immobilization to a solid phase. Reactivity occurs against human ESET protein.Cross reactivity with ESET protein from non-human sources is not expected based on a lack of homology (BLAST) with otherknown proteins.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Western blottingshows reactivity specific for human ESET detecting a band at approximately 170 kDa. Specific band detection by western blotis blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with themembrane. Reactivity in other immunoassays is unknown. Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Western blottingshows reactivity specific for human ESET detecting a band at approximately 170 kDa. Specific band detection by western blotis blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with themembrane. Reactivity in other immunoassays is unknown. Dilutions: ELISA 1:10,000 - 1:50,000 WESTERN BLOT 1:1,000 - 1:2,500 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 1.17 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date ofopening. Recommended Dilutions:

Restrictions: For Research Use only

Images

Western blot analysis is shown using antibodies-online's Affinity Purified anti-ESET antibody to detect human ESET present in a 293 whole cell lysate. ~30ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye