Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) antibody

Antigen: Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1)

Synonyms: SAX3, DUTT1, FLJ21882, MGC131599, MGC133277, Gm310, AW494633, AW742721, ROBO1, robo1, sax3, dutt1, MGC122264, roundabout, roundabout-1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129621

Additional Information

Alternative name: ROBO-1

Gene ID: NP_002932 (isoform a)

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1632-1644 of Human ROBO-1.

Format: Liquid (sterile filtered)

Description: ROBO-1 (also called Roundabout homolog 1 precursor and Deleted in U twenty twenty (DUTT)) functions as a receptor for SLIT1 and SLIT2. The SLIT proteins are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. ROBO-1 may also be required for lung development. ROBO-1 is a type I membrane protein. ROBO-1 is a widely expressed protein with the exception of the kidney. Defects in ROBO1 may be a cause of breast and lung cancer. ROBO-1 maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line. Multiple splice variants have been identified for this protein.

Specificity: This affinity purified antibody is directed against human ROBO-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and dog sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of ROBO-1. Cross reactivity with ROBO-1 homologues from other sources has not been determined.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA , immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~181 kDa in size corresponding to ROBO-1 by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:30,000 - 1:160,000 WESTERN BLOT 1:500 - 1:3,000 IMMUNOHISTOCHEMISTRY 2 ug/ml to 10 ug/ml OTHER APPLICATIONS User Optimized

Concentration: 0.88 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Immunohistochemistry. antibodies-online's Affinity Purified anti-ROBO1 antibody was used at a concentration of 5 ug/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.