GLI Family Zinc Finger 3 (GLI3) antibody

Antigen: GLI Family Zinc Finger 3 (GLI3)

Synonyms: PHS, ACLS, GCPS, PAPA, PAPB, PAP-A, PAPA1, PPDIV, Xt, Bph, Pdn, add, AI854843, AU023367, im:7142603, GLI3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129623

Additional Information

Alternative name: Gli-3

Gene ID: NP_000159

Swiss-Prot: P10071

Immunogen: This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to amino acids 41-57 of human Gli-3 protein.

Format: Liquid (sterile filtered)

Description: Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly- syndactyly syndrome (GCPS), an autosomal dominant disorder-affecting limb and cranio- facial development. Two isoforms of human Gli-3 have been reported. One is the full- length protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.

Specificity: This affinity-purified antibody is directed against human Gli-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail based on 100% sequence homology with the immunogen. Reactivity is also expected against homologues from mouse (94%) and rat (88%) based on partial homology. Reactivity with Gli-3 from other sources is not known.

Application Details

Application Notes: This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot. Dilutions: ELISA 1:6,000 - 1:30,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY 0.5 ug/ml - 5 ug/ml OTHER APPLICATIONS User Optimized

Concentration: 0.84 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.1% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2.

Storage: Store vial at -20° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Research Area: Growth Factors

Restrictions: For Research Use only

Images

Western blot using antibodies-online's anti-Gli-3 antibody shows detection of multiple bands in human lung lysate believed to be Gli-3. Lanes contain 20 ug of whole cell lysates from 1 - human brain, 2 - human lung, 3 - human spleen, 4 - mouse brain and 5 - mouse lung. While no recognizable staining can be seen on mouse tissue, human lung shows what may be truncated Gli-3 (~80kDa). This identity of the strong band at ~50 kDa is unknown. After blocking, the membrane was probed with the primary antibody diluted to 1:500. For detection use HRP Gt-a-Rabbit IgG (ABIN102010). Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Immunohistochemistry. antibodies-online's Affinity Purified anti-Human Gli-3 antibody was used at a 0.625 ug/ml to detect Gli-3 in a variety of tissues. Strong nuclear and smooth muscle staining was noted to be consistent with previously published reports. Specific staining was noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus. This image shows Gli-3 staining of human glioblastoma. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.