RAP1 Interacting Factor Homolog (Yeast) (RIF1) antibody

Antigen: RAP1 Interacting Factor Homolog (Yeast) (RIF1)

Synonyms: AU016181, AW549474, D2Ertd145e, 5730435J01Rik, 6530403D07Rik, FLJ12870, DKFZp434D1026, DKFZp781N1478, RGD1562474, RIF1, fc38c03, wu:fc38c03, si:dkey-89p3.2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129646

Additional Information

Alternative name: Rif1

Swiss-Prot: Q7TPD8

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids 2406-2419 of Mouse Rif1.

Format: Liquid (sterile filtered)

Description: Rif1 (also known as telomere-associated protein RIF1 and Rap1-interacting factor 1) is a mouse ortholog of the yeast Rif1 family of telomere-associated proteins identified on the basis of its high expression in primordial germ cells and embryo-derived pluripotent stem cell lines. Mouse Rif1 is a protein involved in negative regulation of telomere length. It is also localized to ATM dependent DNA damage foci and may act at the chromatin level to allow access to some factors.

Specificity: This affinity purified antibody is directed against mouse Rif1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with Rif1 from human, chimpanzee, rat and dog as only a single amino acid residue change (92% homology) is found for the immunogen sequence. Cross reactivity with Rif1 homologues from other sources has not been determined. Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 kDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 kDa) and mouse Rap1- interacting factor 1 (2426 267.1 kDa).

Application Details

Application Notes: Recommended Dilutions: ELISA 1:5,000 - 1:40,000 WESTERN BLOT 1:500 - 1:3,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 265 kDa in size corresponding to Rif1 by western blotting in the appropriate cell lysate or extract.

Concentration: 1.6 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. Figure. Western blot using Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse R(arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubawith the immunizing peptide (data not shown).Approximately 25 µg of MEF whole cell lysawas separated by SDS-PAGE and transfeonto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 µg/ml for 2 h at room temperature followed by washes and reactio with a 1:10,000 dilution of IRDyeif1 ted te rred n re. TM800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperatuIRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging Systemdeveloped by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. 100 130 170

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only