SPANX Family, Member C (SPANXC) antibody

Antigen: SPANX Family, Member C (SPANXC)

Synonyms: C, CTp11, CT11.3, SPANX-C

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129672

Additional Information

Alternative name: SPANX

Gene ID: CAD29839, CAB75344, CAI41662

Swiss-Prot: Q9NY87

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with asynthetic peptide corresponding to amino acids 31-47 of human SPANX-C protein.

Format: Liquid (sterile filtered)

Description: HumanSperm Proteins Associated with theNucleus on X-chromosome (SPANX)are relatively low molecular weightcytoplasmic proteins found in testisand sperm. Their expression in othertissues indicates malignancies. Familymembers are observed as proteinsthat range from 15 to 20 kDa.

Specificity: This affinity-purified antibody is directed against human SPANX proteins. The immunogen sequenceis present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E. The productwas affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest crossreactivity with SPANX proteins from gorilla and chimpanzee. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: This affinitypurified antibody has been tested foruse in ELISA and by western blot.Specific conditions for reactivity shouldbe optimized by the end user. Expect a band approximately 15-20 kDa in size corresponding to SPANX family members by western blotting in the appropriate humantissue. Most human cell lines are SPANX negative. Dilutions: ELISA 1:10,000 - 1:50,000 WESTERN BLOT 1:500 - 1:3,000 IF MICROSCOPY User Optimized OTHER APPLICATION User Optimized

Concentration: 0.92 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date ofopening.

Research Area: Cancer

Restrictions: For Research Use only