Phosphotyrosine antibody (HRP)

Details for Product No. ABIN135060
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Antigen
Reactivity
Chemical
(165), (49), (32), (28), (16), (10), (8), (8), (4), (1), (1), (1), (1)
Host
Mouse
(188), (48), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
(28), (20), (18), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(171), (110), (93), (34), (33), (29), (29), (16), (15), (10), (3), (1), (1), (1)
Pubmed 2 references available
Quantity 1 mL
Options
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Request Want additional data for this product?

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Catalog No. ABIN135060
286.00 $
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Clone PY20
Isotype IgG2b
Characteristics The antibody detects: Phosphorylated tyrosine and phenylphosphate
Purification Purified
Target Type Chemical
Background Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes Working Dilution:
ELISA: 1:1,000-1:4,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Sample Volume 1 mL
Restrictions For Research Use only
Format Liquid
Buffer Product is supplied as 1.0 mL of stock solution in 50 % glycerol / 50 % PBS, pH 7.4.
Handling Advice Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
General Takagi, Daibata, Last et al.: "Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells." in: The Journal of experimental medicine, Vol. 174, Issue 2, pp. 381-8, 1991 (PubMed).

Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).

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