CD44 antibody

Catalog No. ABIN135069

The Rat Monoclonal anti-CD44 antibody (Clone KM201) (ABIN135069) specifically detects CD44 in FACS, WB, IHC (p), IP, IHC (fro), ICC, BR and Adh Act. The antibody is reactive with Mouse samples.

Quick Overview for CD44 antibody (ABIN135069)

Target: CD44

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Blocking Reagent (BR), Adhesion Activation (Adh Act)

Clone: KM201

Product Details anti-CD44 Antibody

Purpose: Rat Anti-Mouse CD44-LE/AF

Specificity: Mouse CD44 (all isoforms), Mr 80-95 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD44 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, immunoprecipitation, blocking, and adhesion assays.

Endotoxin Level: Low Endotoxin

Immunogen: (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal clone BMS2

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.

Gene ID: 12505

UniProt: P15379

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling