CD22 antibody

Catalog No. ABIN135163

This Rat Monoclonal antibody specifically detects CD22 in FACS, IP, ICC, IHC (p), IHC (fro) and Act. It exhibits reactivity toward Mouse.

Quick Overview for CD22 antibody (ABIN135163)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Activation (Act)

Clone: 2D6

Product Details anti-CD22 Antibody

Purpose: Rat Anti-Mouse CD22-LE/AF

Specificity: Mouse CD22, Mr 150 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD22 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, and activation assays.

Endotoxin Level: Low Endotoxin

Immunogen: Splenic lymphocyte plasma membranes from CBA x C57 F1 mice

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface concomitant with IgD, and is found on most IgM+IgD+ mature B lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via cross-linking of surface Ig increases CD22 expression. CD22 associates with the B cell receptor (BCR) complex and mediates intercellular adhesion. Its intracellular domain is phosphorylated after antigen receptor cross-linking and is involved in negative regulation of B-cell activation.

Gene ID: 12483

UniProt: P35329