CD40 antibody

Catalog No. ABIN135214

This Rat Monoclonal antibody specifically detects CD40 in FACS, IP, BR and Act. It exhibits reactivity toward Mouse.

Quick Overview for CD40 antibody (ABIN135214)

Target: CD40

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD40 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR), Activation (Act)

Clone: 1C10

Product Details anti-CD40 Antibody

Purpose: Rat Anti-Mouse CD40-LE/AF

Specificity: Mouse CD40, Mr 45-50 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD40 antibody for use in flow cytometry, immunoprecipitation, activation, and blocking assays.

Endotoxin Level: Low Endotoxin

Immunogen: sCD40

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: CD40 is a type I cell surface protein belonging to the tumor necrosis factor superfamily of cell surface receptors. In mice it is expressed on B lineage cells, follicular dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zone of secondary lymphoid organs. CD40 first becomes detectable on a subset of small pre-B II cells in bone marrow, with the levels of CD40 expression increasing thereafter during B-cell maturation. Immature B cells (IgM+IgDloB220lo) express intermediate levels of CD40, whereas mature B cells (IgM+IgDhiB220hi) express high levels. CD40 has a central role in B cell growth and differentiation, and signalling through CD40 in combination with IL-4 reportedly induces immunoglobulin isotype switching and secretion of IgE.

Gene ID: 21939

UniProt: P27512

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints