Lysosomal-Associated Membrane Protein 1 (LAMP1) antibody

Antigen: Lysosomal-Associated Membrane Protein 1 (LAMP1)

Synonyms: LAMPA, CD107a, LGP120

Clonality: Monoclonal (1D4B)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN135442

Additional Information

Alternative name: CD107a/LAMP-1

Immunogen: Plasma membrane fraction of mouse embryo NIH3T3 cell line1

Isotype: IgG2a

Clone: 1D4B

Description: Murine CD107a/LAMP-1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa) CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles.1 It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion.2 CD107a is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice.3 LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Purified (1 ug/10^6 cells), Fluorescein (1 ug/10^6 cells), Allophycocyanin (0.2 ug/10^6 cells). Since applications vary, each investigator must determine the optimum working dilutions of the product that is appropriate for their specific needs.

Purification: Purified

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Chen, Pan, DSouza et al.: "Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells." in: Archives of biochemistry and biophysics, Vol. 239, Issue 2, pp. 574-86, 1985 (PubMed).

Rohrer, Schweizer, Russell et al.: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, 1996 (PubMed).

Product: Song, Lee, Schindler: "Deletion of the murine scavenger receptor CD68." in: Journal of lipid research, Vol. 52, Issue 8, pp. 1542-50, 2011 (PubMed).