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LAMP1 antibody (Lysosomal-Associated Membrane Protein 1)

Details for Product anti-LAMP1 Antibody No. ABIN135442, Supplier: Log in to see
Antigen
  • AI196048
  • CD107a
  • Lamp-1
  • LAMPA
  • LGP120
  • LEP100
Reactivity
Mouse (Murine)
256
122
82
24
14
8
5
3
2
Host
Rat
190
103
56
2
Clonality (Clone)
Monoclonal ()
Conjugate
This LAMP1 antibody is un-conjugated
42
31
27
16
7
6
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
178
165
73
73
73
64
44
33
27
19
14
12
4
3
3
2
2
1
1
1
1
1
1
Supplier
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Immunogen Plasma membrane fraction of mouse embryo NIH3T3 cell line1
Clone 1D4B
Isotype IgG2a
Characteristics The antibody detects: Murine CD107a/LAMP-1 (Mr 110-140 kDa with a core protein of Mr approx. 40 kDa)
Purification Purified
Alternative Name CD107a/LAMP-1 (LAMP1 Antibody Abstract)
Background CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107a is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice. LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases.
Pathways
Application Notes Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

Immunoelectron microscopy, Immunoaffinity chromatography

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Product cited in: Song, Lee, Schindler: "Deletion of the murine scavenger receptor CD68." in: Journal of lipid research, Vol. 52, Issue 8, pp. 1542-50, 2011 (PubMed).

Background publications Rohrer, Schweizer, Russell et al.: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, 1996 (PubMed).

Chen, Pan, DSouza et al.: "Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells." in: Archives of biochemistry and biophysics, Vol. 239, Issue 2, pp. 574-86, 1985 (PubMed).