CD28 (CD28) antibody

Antigen: CD28 (CD28)

Synonyms: Tp44, MGC138290

Clonality: Monoclonal (AV7)

Host: Mouse

Reactivity: Chicken, Bird (Avian)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Catalog no.: ABIN135532

Additional Information

Alternative name: CD28

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: AV7

Description: Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50% amino acid sequence identity with mammalian CD28 Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral + T cells are CD28-negative.1 Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both V1- and V2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3.2 MAb AV7 also reacts with turkey CD28 as demonstrated by flow cytometry and immunoprecipitation/Western blotting.

Application Details

Application Notes: WORKING DILUTIONS Flow Cytometry:Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added, store at 2-8C. The FITC conjugate is supplied as 0.5 mg in 1.0 mL of phosphate buffered saline, pH 7.4, containing 0.1% sodium azide as preservative (PBS/NaN3), store at 2-8C. The BIOT conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3, store at 2-8C. The R-PE conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent, store at 2-8C. All products should be stored at 2-8C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Immunoconjugates contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis

Restrictions: For Research Use only

Publications

Publications: Arstila, Vainio, Lassila: "Evolutionarily conserved function of CD28 in alpha beta T cell activation." in: Scandinavian journal of immunology, Vol. 40, Issue 3, pp. 368-71, 1994 (PubMed).

Young, Davison, Tregaskes et al.: "Monomeric homologue of mammalian CD28 is expressed on chicken T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 3848-51, 1994 (PubMed).

Cha, Khatri, Mutnal et al.: "Pathogenic and immunogenic responses in turkeys following in ovo exposure to avian metapneumovirus subtype C." in: Veterinary immunology and immunopathology, 2010 (PubMed).