CD28 antibody (Internal Region)
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- Target See all CD28 Antibodies
- CD28
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This CD28 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- CD28
- Sequence
- SQQLQVYSKT GFNCD
- Specificity
- The immunizing peptide corresponds to a part of the extracellular domain.
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- SQQLQVYSKTGFNCD
- Isotype
- IgG
- Top Product
- Discover our top product CD28 Primary Antibody
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anti-CD28 (CD28) (AA 1-120) antibody
CD28 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Host: Rabbit Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 FITC
anti-CD28 (CD28) (AA 19-152) antibodyCD28 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM61 unconjugated Recombinant Antibody
anti-CD28 (CD28) antibodyCD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibodyCD28 Reactivity: Rat FACS, IP Host: Mouse Monoclonal JJ319 unconjugated
anti-CD28 (CD28) (C-Term) antibodyCD28 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated
anti-CD28 (CD28) antibody (PE)CD28 Reactivity: Rat FACS Host: Mouse Monoclonal JJ319 PE
anti-CD28 (CD28) antibody (PerCP)CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 PerCP
anti-CD28 (CD28) antibody (PE)CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 PE
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 1-2 μg/mL.
Western Blot: Approx 48 kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 25.1 kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL. Primary incuba
Peptide ELISA: antibody detection limit dilution 1:64000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- CD28
- Alternative Name
- CD28 (CD28 Products)
- Synonyms
- Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody
- Background
- CD28, CD28 Molecule, MGC138290, Tp44, CD28 antigen, CD28 antigen (Tp44), T-cell-specific surface glycoprotein
- Gene ID
- 940
- NCBI Accession
- NP_006130
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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