CD44 (CD44) antibody

Antigen: CD44 (CD44)

Synonyms: IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CDW44, CSPG8, HCELL, MUTCH-I, ECMR-III, MGC10468

Clonality: Monoclonal (AV6)

Host: Mouse

Reactivity: Chicken, Bird (Avian)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Catalog no.: ABIN135598

Additional Information

Alternative name: CD44

Format: Purified

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: AV6

Description: Chicken CD44 (Mr 90-kDa). Chicken CD44 is a type I transmembrane glycoprotein expressed on many cell types, including B cells, most T cells, monocytes and some epithelial cells.1 CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development.2,3 MAb AV6 also reacts with IgM+ turkey B cells and a subset of turkey T lymphocytes.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Purified (1 ug/10^6 cells), Fluorescein (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells), Allophycocyanin (0.2 ug/10^6 cells), Spectral Red (0.2 ug/10^6 cells). Since applications vary, investigators are advised to determine the optimum working dilution of the product that is appropriate for a specific application.

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Research Area: Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle

Restrictions: For Research Use only

Publications

Publications: Pure, Camp, Peritt et al.: "Defective phosphorylation and hyaluronate binding of CD44 with point mutations in the cytoplasmic domain." in: The Journal of experimental medicine, Vol. 181, Issue 1, pp. 55-62, 1995 (PubMed).