Mouse anti-Human Immunoglobulin G1 (IgG1) (hinge) Antibody

Antigen: Immunoglobulin G1 (IgG1)

Epitope: hinge

Clonality: Monoclonal (4E3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Catalog no.: ABIN135622

Additional Information

Alternative name: IgG1 (hinge)

Format: Purified

Isotype: IgG1

Clone: 4E3

Description: Reacts with the hinge region of the heavy chain of human IgG1 as demonstrated by ELISA

Application Details

Application Notes: WORKING DILUTIONS Immunofluorescence: Purified (1 ug/10^6 cells), FITC (1 ug/10^6 cells), R-PE (0.1 ug/10^6 cells). ELISA: Purified antibody (under10 ug/mL ), AP (1:500-1:1,000),HRP (1:1,000-1:2,000), BIOT (1:1,000-1:2,000). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Concentration: 0.5 mg/ml

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8C or long-term at -20C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8C or long-term at -20C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Publications: Xu, Chen, Cui et al.: "Influence of myeloperoxidase by anti-myeloperoxidase antibodies and its association with the disease activity in microscopic polyangiitis." in: Rheumatology (Oxford, England), Vol. 49, Issue 11, pp. 2068-75, 2010 (PubMed). Method employed by authors: ELISA

Jackson, Lucas, Melancon et al.: "Clinical relevance and IgG subclass determination of non-HLA antibodies identified using endothelial cell precursors isolated from donor blood." in: Transplantation, Vol. 92, Issue 1, pp. 54-60, 2011 (PubMed).

Xu, Chen, Zhao: "High potential to reverse the inhibition of myeloperoxidase by ceruloplasmin of anti-myeloperoxidase autoantibodies of IgG3 subclass." in: Autoimmunity, 2011 (PubMed).