CD44 (CD44) antibody

Antigen: CD44 (CD44)

Synonyms: IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CDW44, CSPG8, HCELL, MUTCH-I, ECMR-III, MGC10468

Clonality: Monoclonal (F10-44-2)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Blocking Antibody (Inhibition)

Catalog no.: ABIN135705

Additional Information

Alternative name: CD44

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: F10-44-2

Description: Human CD44 CD44, also known as phagocytic glycoprotein-1 (Pgp-1) and the receptor for hyaluronate, exists as a large number of different isoforms resulting from alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells and erythrocytes is a glycosylated type I transmembrane protein. Other variable isoforms containing glycosaminoglycans have molecular weights ranging from 110 to 250 kDa and are widely expressed on hematopoietic and non-hematopoietic cells. CD44 contributes to the adhesion of leukocytes to endothelial cells, stromal cells and the extracellular matrix. It also plays a functional role in cell migration, lymphocyte homing and adhesion during hematopoiesis and lymphocyte activation.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Purified (UNLB) (1 ug/10^6 cells), Fluorescein (10 L/10^6 cells), Biotin (10 L/10^6 cells), R-phycoerythrin (10 L/10^6 cells), Allophycocyanin (10 L/10^6 cells), R-phycoerythrin-Cyanine 7 (10 L/10^6 cells),

Storage: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The R-phycoerythrin-Cyanine 7 (R-PE-CYTM7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed. WARNING Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Research Area: Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle

Restrictions: For Research Use only