Selectin E (SELE) antibody

Antigen: Selectin E (SELE)

Synonyms: ELAM, ESEL, CD62E, ELAM1, LECAM2, SELE

Clonality: Monoclonal (1-2B6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), ELISA, Blocking Antibody (Inhibition)

Catalog no.: ABIN135713

Additional Information

Alternative name: CD62E/E-selectin

Isotype: IgG1

Clone: 1-2B6

Description: Human CD62E/E-selectin CD62E represents the 100-110 kDa type I transmembrane glycoprotein E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). It is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial attachment and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Purified (UNLB) (1 ug/10^6 cells), Fluorescein (10 L/10^6 cells), BIOT (10 L/10^6 cells), R-phycoerythrin (10 L/10^6 cells), Spectral RedTM (10 L/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Purification: Ion exchange

Storage: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of phosphate buffered saline containing 0.1% PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) and Spectral RedTM (SPRD) are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed. WARNING Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Gearing, Newman: "Circulating adhesion molecules in disease." in: Immunology today, Vol. 14, Issue 10, pp. 506-12, 1994 (PubMed).

Tedder, Steeber, Chen et al.: "The selectins: vascular adhesion molecules." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 9, Issue 10, pp. 866-73, 1995 (PubMed).