Vascular Cell Adhesion Molecule 1 (VCAM1) antibody (Biotin)

Antigen: Vascular Cell Adhesion Molecule 1 (VCAM1)

Synonyms: CD106, MGC99561, INCAM-100, DKFZp779G2333, Vcam-1, VCAM1B, MGC108734, VCAM1, fi76d06, wu:fi76d06, zgc:158875, VCAM-1, DKFZp469N1820

Clonality: Monoclonal (1-G11B1)

Host: Mouse

Conjugate: Biotin

Catalog no.: ABIN135718

Additional Information

Alternative name: CD106/VCAM-1

Isotype: IgG1

Clone: 1-G11B1

Description: Human CD106/VCAM-1 CD106, also known as INCAM-1, is a 100-110 kDa vascular adhesion cell adhesion molecule-1 (VCAM-1) that is member of the immunoglobulin superfamily. CD106 is expressed predominantly on cytokine-activated vascular endothelium but has also been identified on interfollicular dendritic cells, some macrophages and bone marrow stromal cells. Endothelial CD106 binds the integrins 41 (CD49d/CD29, VLA-4) and 47 and contributes to extravasation of lymphocytes, monocytes, basophils and eosinophils (but not neutrophils) from blood vessels, particularly at sites of inflammation. Unlike the 2 integrins, the CD106-VLA-4 interaction can mediate both the initial tethering and rolling of lymphocytes on endothelium as well as their subsequent arrest and firm adhesion. CD106 expressed on non-vascular tissues has been implicated in the interaction of hematopoietic progenitors with bone marrow stromal cells, B cell binding to follicular dendritic cells, costimulation of T cells, and embryonic development. The monoclonal antibody 1.G11B1 inhibits in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Purified (UNLB) (1 ug/10^6 cells), Fluorescein (10 L/10^6 cells), Biotin (10 L/10^6 cells), R-phycoerythrin (10 L/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Restrictions: For Research Use only

Publications

Publications: Thornhill, Wellicome, Mahiouz et al.: "Tumor necrosis factor combines with IL-4 or IFN-gamma to selectively enhance endothelial cell adhesiveness for T cells. The contribution of vascular cell adhesion molecule-1-dependent and -independent binding mechanisms." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 2, pp. 592-8, 1991 (PubMed).

Bevilacqua: "Endothelial-leukocyte adhesion molecules." in: Annual review of immunology, Vol. 11, pp. 767-804, 1993 (PubMed).