CD4 antibody (FITC)

Catalog No. ABIN135726

This anti-CD4 antibody is a Mouse Monoclonal antibody detecting CD4 in FACS. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for CD4 antibody (FITC) (ABIN135726)

Target: CD4

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: RFT4

Product Details anti-CD4 Antibody (FITC)

Specificity: Human CD4, Mr 55 kDa

Characteristics: Mouse Anti-Human CD4-FITC

Immunogen: Thymocyte and E-rosetted lymphocytes

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , WB - Reported in literature , Sep - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, PE/TXRD, APC, SPRD, CY5, PE/CY5.5, 10 L/106 cells PE/CY7, APC/CY7, AF488, AF647, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Buffer: 25 tests in 0.25 mL or 100 tests in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD4 antibody (FITC) (ABIN135726)

Rhead, Brorson, Berge, Adams, Quach, Moen, Berg-Hansen, Celius, Sangurdekar, Bronson, Lea, Burnard, Maltby, Scott, Lechner-Scott, Harbo, Bos, Barcellos: "Increased DNA methylation of SLFN12 in CD4+ and CD8+ T cells from multiple sclerosis patients." in: PLoS ONE, Vol. 13, Issue 10, pp. e0206511, (2019) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: D4 is a 59 kDa type I transmembrane glycoprotein and a member of the Ig-SF of cell surface receptors. It is expressed predominantly on the helper/inducer subpopulation of mature T lymphocytes, and on monocytes and macrophages. Domains 1 and 2 bind to MHC Class II antigens, while domains 3 and 4 may be involved in cis interactions with the CD3/TCR complex. CD4 functions as an accessary molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events