FAS antibody

Catalog No. ABIN135817

This Mouse Monoclonal antibody specifically detects FAS in FACS, ELISA, IP, ICC, IHC (fro), IHC (p) and AD. It exhibits reactivity toward Human, Rhesus Monkey, Sooty mangabey (Cercocebus atys) and Green Monkey.

Quick Overview for FAS antibody (ABIN135817)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human, Rhesus Monkey, Sooty mangabey (Cercocebus atys), Green Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Apoptosis Detection (AD)

Clone: DX2

Product Details anti-FAS Antibody

Purpose: Mouse Anti-Human CD95-LE/AF

Specificity: Human/Rhesus/African Green Monkey/Sooty Mangabey CD95, Mr 45 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Human CD95 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, ELISA, and apoptosis assays.

Endotoxin Level: Low Endotoxin

Immunogen: Human CD95 transfected L cells

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95

Background: CD95, also known as Fas and Apo-1, is a 40-50 kDa type I transmembrane glycoprotein and a member of the tumor necrosis factor receptor superfamily. It is expressed by activated lymphocytes, monocytes, neutrophils, fibroblasts and cell lines. Fas ligand binding to CD95 induces apoptosis in activated mature lymphocytes thereby playing a role in maintaining peripheral tolerance. Crosslinking of CD95 by the monoclonal antibodies DX2 and DX3 delivers an apoptotic signal to Fas-sensitive cells, indicating that these monoclonal antibodies recognize a functional epitope of CD95.

Gene ID: 355, 574332, 103216224

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity